个人简介

严明，副教授，硕士生导师。1996年于南京大学获生物化学理学硕士学位，1997-2000年在南京军区南京总医院检验中心工作，2001年至今在999策略手机论坛网址从事生物信息学与微生物应用技术的科研和教学工作，作为生物信息学研究室负责人，在蛋白质工程和代谢工程等领域的应用研究积累了丰富的经验。近年来参加了国家973项目“新一代生物催化与生物转化的科学基础”和“人工合成细胞工厂”等多项国家与省部级研究项目。在国内外检索期刊上发表论文30余篇，其中SCI论文10余篇，申请国家发明专利10余项，译有《比较基因组学手册：原理与方法》一书。

研究方向

1、酶的结构生物学与生物信息学

借助结构生物学和生物信息学技术深入研究重要工业酶的序列、结构和功能关系，在对酶蛋白三维空间结构进行解析的基础上，通过对酶蛋白进行构效解析、理性设计和定向进化，提出理性设计和定向进化策略来生产新型和改良工业酶。

2、精细化学品的生物催化与转化

开展工业生物催化剂及其催化过程的研究，采用酶或全细胞催化技术，生产化学法难以转化与合成的新型生物产品，或替代现行化学合成工艺中的复杂步骤。研究生物体及其酶进行定向物质转化的分子机制，开发新的生物合成技术。开发化工原料、医药原料、手性化合物、功能糖与糖基化学品等高值精细化学品的高效生物合成技术，以生物技术改造化学工业过程。

代表性成果

1.  Yang X, Lei G, Ying H, Xu L, Yan M: Enhancing the production of uridine 5'-monophosphate by recombinant Saccharomyces cerevisiae using a whole cell biocatalytic process. World Journal of Microbiology and Biotechnology 2011:1-7.

2. Liu H, Liu K, Yan M, Xu L, Ouyang P: gTME for Improved Adaptation of Saccharomyces cerevisiae to Corn Cob Acid Hydrolysate. Applied Biochemistry and Biotechnology 2011:1-10.

3. Ye Q, Li X, Yan M, Cao H, Xu L, Zhang Y, Chen Y, Xiong J, Ouyang P, Ying H: High-level production of heterologous proteins using untreated cane molasses and corn steep liquor in Escherichia coli medium. Applied microbiology and biotechnology 2010, 87(2):517-525.

4. Ye Q, Cao H, Zang G, Mi L, Yan M, Wang Y, Zhang Y, Li X, Li J, Xu L: Biocatalytic synthesis of (S)-4-chloro-3-hydroxybutanoate ethyl ester using a recombinant whole-cell catalyst. Applied microbiology and biotechnology 2010:1-9.

5. Ye Q, Cao H, Yan M, Cao F, Zhang Y, Li X, Xu L, Chen Y, Xiong J, Ouyang P: Construction and co-expression of a polycistronic plasmid encoding carbonyl reductase and glucose dehydrogenase for production of ethyl (S)-4-chloro-3-hydroxybutanoate. Bioresource technology 2010, 101(17):6761-6767.

6. Ye Q, Cao H, Mi L, Yan M, Wang Y, He Q, Li J, Xu L, Chen Y, Xiong J: Biosynthesis of (S)-4-chloro-3-hydroxybutanoate ethyl using Escherichia coli co-expressing a novel NADH-dependent carbonyl reductase and a glucose dehydrogenase. Bioresource technology 2010.

7.Yao T, Xu L, Ying H, Huang H, Yan M: The catalytic property of 3-hydroxyisobutyrate dehydrogenase from Bacillus cereus on 3-hydroxypropionate. Applied Biochemistry and Biotechnology 2010, 160(3):694-703.

8. Yang X, Xu L, Yan M: Modeling metabolic response to changes of enzyme amount in yeast glycolysis. African Journal of Biotechnology 2010, 9(41):6894-6901.

9. Liu H, Yan M, Lai C, Xu L, Ouyang P: gTME for improved xylose fermentation of Saccharomyces cerevisiae. Applied Biochemistry and Biotechnology 2010, 160(2):574-582.

10. Hao N, Yan M, Zhou H, Liu H, Cai P, Ouyang P: The effect of AmtR on growth and amino acids production in Corynebacterium glutamicum. Applied Biochemistry and Microbiology 2010, 46(6):561-566.

11. Hao N, Li Y, Yan M, Ouyang PK: Prediction and analysis of the secreteomic in Corynebacterium glutamicum ATCC 13032. African Journal of Biotechnology 2010, 9(11):1561-1567.

12. Cao H, Mi L, Ye Q, Zang G, Yan M, Wang Y, Zhang Y, Li X, Xu L, Xiong J et al: Purification and characterization of a novel NADH-dependent carbonyl reductase from Pichia stipitis involved in biosynthesis of optically pure ethyl (S)-4-chloro-3-hydroxybutanoate. Bioresource technology 2010.

13. Ye Q, Yan M, Yao Z, Xu L, Cao H, Li Z, Chen Y, Li S, Bai J, Xiong J: A new member of the short-chain dehydrogenases/reductases superfamily: Purification, characterization and substrate specificity of a recombinant carbonyl reductase from Pichia stipitis. Bioresource technology 2009, 100(23):6022-6027.

14. Ye Q, Yan M, Xu L, Cao H, Li Z, Chen Y, Li S, Ying H: A novel carbonyl reductase from Pichia stipitis for the production of ethyl (S)-4-chloro-3-hydroxybutanoate. Biotechnology letters 2009, 31(4):537-542.

15. Xu W, Yan M, Xu L, Ding L, Ouyang P: Engineering the activity of thermophilic xylose isomerase by site-directed mutation at subunit interfaces. Enzyme and Microbial Technology 2009, 44(2):77-83.

16. Xu W, Cai P, Yan M, Xu L, Ouyang P: Molecular Dynamics Simulation of Temperature-dependent Flexibility of Thermophilic Xylose Isomerase. Chinese Journal of Chemical Physics 2009, 22:467.

17. XU W, CAI P, YAN M, XU L, OUYANG PK: Molecular docking of xylitol and xylose isomerase from Thermus thermophilus and model analysis. Chem J Chin Univ 2007, 28:971-973.

18. Li Y, Yan M, Wang K, Xu L, Xu W, Ouyang P: Study on dsRNA Mediated Gene Silencing in Saccharomyces cerevisiae: Suppressing the Expression of GRE3 Gene. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 2007, 34(3):292-298.